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Anti-PRKCB Antibody Y125
Also for PRKCB (NM_002738)
|A synthetic peptide corresponding to residues near C-terminus of human PKC beta-II was used as immunogen. The antibody does not cross-react with human PKC beta-I. Predicted to cross-react with mouse and bovine PKC beta, rat and pig PKC beta-II, based on s|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:50; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens protein kinase C, beta (PRKCB), transcript variant 2|
|PKC-beta; PKCB; PRKCB1; PRKCB2|
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Wnt Signaling Pathway
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Western blot - PKC beta 2 antibody [Y125]; Anti-PKC beta 2 antibody [Y125] at 1/500 dilution + K562 cell lysate.Predicted band size : 77 kDa.Observed band size : 77 kDa.
Immunohistochemistry (Paraffin-embedded sections) - PKC beta 2 antibody [Y125]; ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
Immunofluorescence - PKC beta 2 antibody [Y125]; ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
Flow Cytometry-Anti-PKC beta 2 antibody [Y125](ab32026); Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.