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Anti-PRKCB Antibody Y125
Also for PRKCB (NM_002738)
|A synthetic peptide corresponding to residues near C-terminus of human PKC beta-II was used as immunogen. The antibody does not cross-react with human PKC beta-I. Predicted to cross-react with mouse and bovine PKC beta, rat and pig PKC beta-II, based on s|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:50; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
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Western blot - PKC beta 2 antibody [Y125]; Anti-PKC beta 2 antibody [Y125] at 1/500 dilution + K562 cell lysate.Predicted band size : 77 kDa.Observed band size : 77 kDa.
Immunohistochemistry (Paraffin-embedded sections) - PKC beta 2 antibody [Y125]; TA303830, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
Immunofluorescence - PKC beta 2 antibody [Y125]; TA303830, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
Flow Cytometry-Anti-PKC beta 2 antibody [Y125](TA303830); Overlay histogram showing HeLa cells stained with TA303830 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.