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Home All anti-PRKCB1 antibodies
Anti-PRKCB1 Phospho Antibody EP1902Y
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for PRKCB1 (NM_002738)
OriGene Data
| A phospho specific peptide corresponding to residues surrounding serine 660 of human PKC?II was used as an immunogen. This antibody detects PKC?II phosphorylated at serine 660. |
| EP1902Y |
IgG |
| Human, Mouse, Rat |
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| WB, IHC |
WB: 1: 10,000; IHC: 1: 100; ICC: 1:50 |
| 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| (Protein A or G Sepharose) |
Reference Data
| Homo sapiens protein kinase C, beta (PRKCB), transcript variant 2 |
| PKC-beta; PKCB; PRKCB1; PRKCB2 |
| NP_002729 |
| PKC?II is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKC?II is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKC?II structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. It has been shown that PKC?II must first be processed by three distinct phosphorylations before it is competent to respond to second messengers. Specifically, the threonine at position 500 (T500) on the activation loop, and T641 and S660 on the carboxyl terminus of protein kinase C beta II are phosphorylated in vivo. T500 and S660 are selectively dephosphorylated in vitro by protein phosphatase 2A to yield an enzyme that is still capable of lipid-dependent activation, whereas all three residues are dephosphorylated by protein phosphatase 1 to yield an inactive enzyme. (4). |
EGFR1 Signaling Pathway Focal Adhesion Hemostasis MAPK signaling pathway Melanogenesis TGF Beta Signaling Pathway Wnt Signaling Pathway |
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WB Image
Western blot analysis on Jurkat cell lysates using anti-Phospho- PKCβII (pS660), 1:100,000 dilution. Cells were either (A) untreated (B) treated with AP.
IHC Image
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti- PKCβII.
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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