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Anti-PRKCA PHOSPHO Antibody EP2608Y
Also for PRKCA (NM_002737)
|A phospho specific peptide corresponding to residues surrounding threonine 497 of human PKC alpha was used as an immunogen. This antibody detects PKC alpha phosphorylated on thr497.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:50,000 -100,000; IHC: 1:100 -250; ICC: 1:100 -250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens protein kinase C, alpha (PRKCA)|
|AAG6; PKC-alpha; PKCA; PRKACA|
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - PKC alpha (phospho T497) antibody [EP2608Y]; All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] at 1/100000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate treated with LP.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 77 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC alpha (phospho T497) antibody [EP2608Y]; ab76016, at 1/100 dilution, staining PKC alpha in human kidney by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - PKC alpha (phospho T497) antibody [EP2608Y]; ab76016, at 1/100 dilution, staining PKC alpha in HeLa cells by immunofluorescence.