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Anti-PRKCA PHOSPHO Antibody EP2608Y
Also for PRKCA (NM_002737)
|A phospho specific peptide corresponding to residues surrounding threonine 497 of human PKC alpha was used as an immunogen. This antibody detects PKC alpha phosphorylated on thr497.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:50,000 -100,000; IHC: 1:100 -250; ICC: 1:100 -250; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - PKC alpha (phospho T497) antibody [EP2608Y]; All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] at 1/100000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate treated with LP.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 77 kDa.Observed band size : 80 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC alpha (phospho T497) antibody [EP2608Y]; TA303660, at 1/100 dilution, staining PKC alpha in human kidney by immunohistochemistry using paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - PKC alpha (phospho T497) antibody [EP2608Y]; TA303660, at 1/100 dilution, staining PKC alpha in HeLa cells by immunofluorescence.