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Anti-PRKCA Antibody Y143
Also for PRKCA (NM_002737)
|A synthetic peptide corresponding to residues near the N-terminus of human PKC a was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:50; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - PKC alpha antibody [Y143]; Anti-PKC alpha antibody [Y143] at 1/2000 dilution + K562 cell lysate.Predicted band size : 77 kDa.Observed band size : 77 kDa.Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha antibody [Y143]; TA303659, staining human carcinoma of thyroid gland by immunohistochemistry, Paraffin embedded tissue
Immunofluorescence - PKC alpha antibody [Y143]; TA303659, staining of Hela cells by immunofluorescent
Flow Cytometry - Anti-PKC alpha antibody [Y143]; Overlay histogram showing HeLa cells stained with TA303659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.