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Anti-PRKCA Antibody Y143
Also for PRKCA (NM_002737)
|A synthetic peptide corresponding to residues near the N-terminus of human PKC a was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:2000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:50; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens protein kinase C, alpha (PRKCA)|
|AAG6; PKC-alpha; PKCA; PRKACA|
Entrez Gene 5578 Human
Entrez Gene 18750 Mouse
Entrez Gene 24680 Rat
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]. |
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - PKC alpha antibody [Y143]; Anti-PKC alpha antibody [Y143] at 1/2000 dilution + K562 cell lysate.Predicted band size : 77 kDa.Observed band size : 77 kDa.Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - PKC alpha antibody [Y143]; TA303659, staining human carcinoma of thyroid gland by immunohistochemistry, Paraffin embedded tissue
Immunofluorescence - PKC alpha antibody [Y143]; TA303659, staining of Hela cells by immunofluorescent
Flow Cytometry - Anti-PKC alpha antibody [Y143]; Overlay histogram showing HeLa cells stained with TA303659 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.