OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PRKAR2B antibodies

Anti-PRKAR2B Antibody EP2649Y

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA301234
  • Rabbit Monoclonal Antibody against PRKAR2B (Clone EP2649Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419138) , 20ug Explanation
100ul $325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
IHC(1)
FC(1)
spacer
Also for PRKAR2B (NM_002736)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human PKA RIIb was used as an immunogen.
Clone NameEP2649Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:25,000~50,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:80; IP: 1:60
Predicted MW Explanation 46 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type II, beta (PRKAR2B)
Alternative NamePRKAR2; RII-BETA
Database LinkNP_002727
Entrez Gene 5577 Human
Entrez Gene 19088 Mouse
Entrez Gene 24679 Rat
FunctionCyclic AMP is an important second messenger in the coordinated regulation of cellular metabolism. Its effects are mediated by cAMP-dependent protein kinase (PKA), which is assembled from two regulatory (R) and two catalytic (C) subunits. The RIIb isoform is abundant in brown and white adipose tissue and brain, with limited expression elsewhere. Results demonstrate a role for the RIIb holoenzyme in regulating energy balance and adiposity (1). Disruption of the RIIb regulatory subunit gene of PKA results in release of the active catalytic subunit and an increase in basal PKA activity in cells where RIIb is highly expressed (2). The cAMP-dependent protein kinase system is believed to mediate most, if not all, cellular effects of the second messenger cAMP. Free, activated C subunits of PKA RIIb catalyze the phosphorylation of specific substrate proteins on serine and threonine residues and thereby alter their activity or function (3).
Related PathwayDruggable Genome ApoptosisInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] at 1/50000 dilution + Human brain lysate at 10 ug.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 46 kDa.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKA 2 beta (regulatory subunit) antibody [EP2649Y]; TA301234, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
spacer
Flow Cytometry-Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y](TA301234); Overlay histogram showing SH-SY5Y cells stained with TA301234 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title