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Anti-PRKAR2A TRUEMAB Antibody Clone OTI1F8
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PRKAR2A (NM_004157)
|Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A)|
Entrez Gene 5576 Human
|cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq].|
|Druggable Genome ApoptosisInsulin signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR2A monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501146)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501146)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501146)
Anti-PRKAR2A mouse monoclonal antibody (TA501146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A(RC220376).
HEK293T cells transfected with either RC220376 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKAR2A antibody(TA501146), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PRKAR2A antibody(TA501146),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PRKAR2A antibody(TA501146),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).