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Anti-PRKAR1B TRUEMAB Antibody Clone OTI2A3
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PRKAR1B (NM_002735)
|Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein kinase cAMP-dependent type I regulatory subunit beta (PRKAR1B), transcript variant 2|
Entrez Gene 5575 Human
|Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009].|
|Druggable Genome ApoptosisInsulin signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1B (RC207809 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
Anti-PRKAR1B mouse monoclonal antibody (TA502750) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B(RC207809 ).
HEK293T cells transfected with either RC207809 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKAR1B antibody(TA502750), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).