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Home Antibody All anti-PRKAR1B antibodies

Anti-PRKAR1B TRUEMAB Antibody Clone OTI2A3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502750 PRKAR1B (PKA regulatory subunit I beta) mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) 100ul $379 In Stock
LC400964 PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502750 Carrier-free (BSA/glycerol-free) PRKAR1B mouse monoclonal antibody, clone OTI2A3 (formerly 2A3) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(2)
IF(1)
FC(3)
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Also for PRKAR1B (NM_002735)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.
Clone NameClone OTI2A3 IsotypeIgG1
Species ReactivityHuman Concentration0.57 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 42.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2
Alternative NamePRKAR1
Database LinkNP_002726
Entrez Gene 5575 Human
FunctionCyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009].
Related PathwayDruggable Genome ApoptosisInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1B (RC207809 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B.
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
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Anti-PRKAR1B mouse monoclonal antibody (TA502750) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B(RC207809 ).
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HEK293T cells transfected with either RC207809 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKAR1B antibody(TA502750), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).
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