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Anti-PRKAG1 Antibody Y308


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300519 Rabbit Monoclonal Antibody against PRKAG1 (Clone Y308) 100ul $325 In Stock
LC400962 PRKAG1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen
Clone NameY308 IsotypeIgG
Species ReactivityMouse, Rat, Human, African Green Monkey ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IP Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:20; IP: Use at an assay dependent concentration
Predicted MW Explanation 38 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for ICC or IHC.

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), transcript variant 1
Alternative NameAMPKG
Database LinkNP_002724
Entrez Gene 5571 Human
Entrez Gene 19082 Mouse
Entrez Gene 25520 Rat
Entrez Gene 709298 Monkey
FunctionThe 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising a (63 kDa), ß (38 kDa) and ? (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).
Related PathwayDruggable Genome Insulin signaling pathwayAdipocytokine signaling pathwayHypertrophic cardiomyopathy (HCM)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - AMPK gamma 1 antibody [Y308]; Anti-AMPK gamma 1 antibody [Y308] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 38 kDa.Observed band size : 38 kDa.
AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonalto AMPK gamma 1and 50ul of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Secondary: Mouse monoclonalSecondary Antibody to Rabbit IgG light chain (HRP) . Bands: 37kDa: AMPK gamma 1.


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