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Anti-PRKAG1 Antibody Y308
Also for PRKAG1 (NM_002733)
|A synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen|
|Mouse, Rat, Human, African Green Monkey
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:20; IP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IHC.
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Western blot - AMPK gamma 1 antibody [Y308]; Anti-AMPK gamma 1 antibody [Y308] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 38 kDa.Observed band size : 38 kDa.
Immunoprecipitation - Anti-AMPK gamma 1 antibody [Y308]; AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50ul of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300519. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) . Bands: 37kDa: AMPK gamma 1.