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Anti-PRKACA Antibody EP2102Y
Also for PRKACA (NM_002730)
|A synthetic peptide corresponding to residues near the C-terminus of human PKC C-alpha subunit was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:50,000 - 200,000; IHC: 1:100; ICC: 1:100 - 250; FC: 1;60; IP: 1;50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; All lanes : Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] at 1/200000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 46 kDa.Observed band size : 42 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; TA303655, at a 1/100 dilution, staining human cAMP Protein Kinase Catalytic Subunit in testis by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]; ICC/IF image of TA303655 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y](TA303655); Overlay histogram showing HeLa cells stained with TA303655 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.