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Anti-PRKAB1 Antibody EP2181Y
Also for PRKAB1 (NM_006253)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:2,000-20,000; IHC: 1: 100; ICC: 1: 100~250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)|
|The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising ? (63 kDa), ? (38 kDa) and ? (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phsopholyation on serine 24/25 and serine 182 has been linked to AMPK localization. |
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Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]; All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] at 1/20000 dilution.Lane 1 : human brain tissue lysate (cells were untreated).Lane 2 : human brain tissue lysate (cells were treated with AP).Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 32 and 45 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 (phospho S181) antibody [EP2181Y]; Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.
Immunocytochemistry/ Immunofluorescence - AMPK beta 1 (phospho S181) antibody [EP2181Y]; Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution.