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Home Antibody All anti-PRKAB1 antibodies

Anti-PRKAB1 Antibody Y367

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300530
  • Rabbit Monoclonal Antibody against PRKAB1 (Clone Y367)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416773) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for PRKAB1 (NM_006253)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen.
Clone NameY367 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; FC: 1:50; IP: 1:80
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)
Alternative NameAMPK; HAMPKb
Database LinkNP_006244
FunctionThe 5’-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non–fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising a (63 kDa), ß (38 kDa) and ? (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non–catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).
Related Pathway

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WB Image
Western blot - AMPK beta 1 antibody [Y367]; All lanes : Anti-AMPK beta 1 antibody [Y367] at 1/5000 dilution.Lane 1 : (A) : NIH 3T3.Lane 2 : (B) : Hela.Lane 3 : (C) : A431.Lane 4 : (D) : PC-12.Predicted band size : 30 kDa.Observed band size : 38 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 antibody [Y367]; TA300530 at a 1:100 dilution staining AMPK beta 1 in human lung carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
FC Image
Flow Cytometry-Anti-AMPK beta 1 antibody [Y367](TA300530); Overlay histogram showing HeLa cells stained with TA300530 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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