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Anti-PRKAA1 PHOSPHO Antibody EPR3051
Also for PRKAA1 (NM_006251)
|A phospho specific peptide corresponding to residues surrounding Serine 487 of human AMPK alpha 1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1|
|The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.|
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Western blot - AMPK alpha 1 (phospho S487) antibody [EPR3051]; All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] at 1/1000 dilution.Lane 1 : 293T cell lysate untreated.Lane 2 : 293T cell lysate treated with LP.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 64 kDa.
Immunocytochemistry/ Immunofluorescence - AMPK alpha 1 (phospho S487) antibody [EPR3051]; Immunofluorescent staining of AMPK alpha 1 in HeLa cells using ab92701 at 1/100 dilution.