OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PRKAA1 antibodies

Anti-PRKAA1 PHOSPHO Antibody EPR3051

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307170
  • Rabbit monoclonal antibody against AMPK alpha 1 Phospho (pS487)(clone EPR3051) (Phospho-Specific)
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IF(1)
spacer
Also for PRKAA1 (NM_006251)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 487 of human AMPK alpha 1 was used as an immunogen.
Clone NameEPR3051 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000 - 10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1
Alternative NameAMPK; AMPKa1
Database LinkNP_006242
FunctionThe 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - AMPK alpha 1 (phospho S487) antibody [EPR3051]; All lanes : Anti-AMPK alpha 1 (phospho S496) antibody [EPR3051] at 1/1000 dilution.Lane 1 : 293T cell lysate untreated.Lane 2 : 293T cell lysate treated with LP.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 64 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - AMPK alpha 1 (phospho S487) antibody [EPR3051]; Immunofluorescent staining of AMPK alpha 1 in HeLa cells using TA307170 at 1/100 dilution.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title