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Anti-PRDX1 Antibody EPR5433
Also for PRDX1 (NM_001202431)
|A synthetic peptide corresponding to residues in human Peroxiredoxin 1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:1000; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens peroxiredoxin 1 (PRDX1), transcript variant 4|
|MSP23; NKEF-A; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2|
|Peroxiredoxin 1 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Peroxiredoxin 1 may play a role in the regulation of phospholipid turnover, as well as in protection against oxidative injury (1).|
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Western blot - Peroxiredoxin 1 antibody [EPR5433]; All lanes : Anti-Peroxiredoxin 1 antibody [EPR5433] at 1/10000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : K562 cell lysates .Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 22 kDa.Observed band size : 22 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 1 antibody [EPR5433]; Immunohistochemical analysis of paraffin-embedded kidney tissue using ab109498 at 1/250
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Peroxiredoxin 1 antibody [EPR5433]; Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissue using ab109498 at 1/250.
Immunocytochemistry/ Immunofluorescence - Peroxiredoxin 1 antibody [EPR5433]; Immunofluorescent analysis of 293T cells labeled with ab109498 at 1/10.
Flow Cytometry - Anti-Peroxiredoxin 1 antibody [EPR5433]; Overlay histogram showing HeLa cells stained with ab109498 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.