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Anti-PRC1 Antibody EP1513Y
Also for PRC1 (NM_003981)
|A synthetic peptide corresponding to residues on human PRC1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:10,000; IHC: 1:100 250; ICC: 1:100 250; FC: 1:20; IP: 1:10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant 1|
Entrez Gene 9055 Human
Entrez Gene 233406 Mouse
Entrez Gene 308761 Rat
|This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. |
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Western blot - PRC1 antibody [EP1513Y]; Anti-PRC1 antibody [EP1513Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Immunohistochemistry (Paraffin-embedded sections) - PRC1 antibody [EP1513Y]; Ab51248 (1:100) staining human PRC1 in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - PRC1 antibody [EP1513Y]; Immunofluorescent staining of HeLa cells using TA303759 (1:100).
Immunocytochemistry/ Immunofluorescence - PRC1 antibody [EP1513Y]; TA303759 staining PRC1 in human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked in 1% serum for 1 hour at 37Â°C without permeation step. The primary antibody was diluted 1/100 (PBS) and incubated with sample for 1 hour at 20Â°C. An Alexa Fluor® 488 conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
Flow Cytometry-Anti-PRC1 antibody [EP1513Y](TA303759); Overlay histogram showing HeLa cells stained with TA303759 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.