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Home Antibody All anti-PRC1 antibodies

Anti-PRC1 Antibody EP1513Y

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SKU Description Amount Price Availability*  
TA303759
  • Rabbit monoclonal antibody against PRC1 (EP1513Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418307) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human PRC1 was used as an immunogen.
Clone NameEP1513Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10,000; IHC: 1:100 250; ICC: 1:100 250; FC: 1:20; IP: 1:10
Predicted MW Explanation 72 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant 1
Alternative NameASE1
Database LinkNP_003972
Entrez Gene 9055 Human
Entrez Gene 233406 Mouse
Entrez Gene 308761 Rat
FunctionThis gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PRC1 antibody [EP1513Y]; Anti-PRC1 antibody [EP1513Y] at 1/10000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
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Immunohistochemistry (Paraffin-embedded sections) - PRC1 antibody [EP1513Y]; Ab51248 (1:100) staining human PRC1 in human cervical carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
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Immunocytochemistry/ Immunofluorescence - PRC1 antibody [EP1513Y]; Immunofluorescent staining of HeLa cells using TA303759 (1:100).
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Immunocytochemistry/ Immunofluorescence - PRC1 antibody [EP1513Y]; TA303759 staining PRC1 in human breast cancer cells by ICC/IF. The cells were paraformaldehyde fixed and blocked in 1% serum for 1 hour at 37°C without permeation step. The primary antibody was diluted 1/100 (PBS) and incubated with sample for 1 hour at 20°C. An Alexa Fluor® 488 conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used as secondary.
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Flow Cytometry-Anti-PRC1 antibody [EP1513Y](TA303759); Overlay histogram showing HeLa cells stained with TA303759 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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