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Anti-PRC1 PHOSPHO Antibody EP1514Y


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against PRC1 (pT481) Phospho (EP1514Y ) (phospho-specific)
100ul 325 3-7 Days
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481.
Clone NameEP1514Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant 3
Alternative NameASE1
Database LinkNP_955446
Entrez Gene 9055 Human
FunctionThis gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PRC1 (phospho T481) antibody [EP1514Y]; All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] at 1/500 dilution.Lane 1 : HeLa cell lysates (untreated).Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labeled, at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
IF Image
Immunocytochemistry - PRC1 (phospho T481) antibody [EP1514Y]; TA303758 at 1/100 dilution staining PRC1 in HeLa cells by Immunocytochemistry.


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