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Home Antibody All anti-PPP3CA antibodies

Anti-PPP3CA Antibody EP1669Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303653
  • Rabbit monoclonal antibody against PP2B (N-term) (EP1669Y )
100ul $325 In Stock
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WB(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus of human PP2B was used as an immunogen.
Clone NameEP1669Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:10000; IP: 1:100; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1
Alternative NameCALN; CALNA; CALNA1; CCN1; CNA1; PPP2B
Database LinkNP_000935
Function
Related Pathway
MAPK signaling pathway
Wnt Signaling Pathway

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WB Image
Western blot - Calcineurin A antibody [EP1669Y]; Anti-Calcineurin A antibody [EP1669Y] at 1/10000 dilution + Hela cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 59 kDa.Observed band size : 59 kDa.
FC Image
Flow Cytometry - Calcineurin A antibody [EP1669Y]; Overlay histogram showing HeLa cells stained with TA303653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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