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Anti-PPP3CA Antibody EP1669Y
Also for PPP3CA (NM_000944)
|A synthetic peptide corresponding to residues on the N-terminus of human PP2B was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000; IP: 1:100; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1|
|CALN; CALNA; CALNA1; CCN1; CNA1; PPP2B|
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot - Calcineurin A antibody [EP1669Y]; Anti-Calcineurin A antibody [EP1669Y] at 1/10000 dilution + Hela cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 59 kDa.Observed band size : 59 kDa.
Flow Cytometry - Calcineurin A antibody [EP1669Y]; Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.