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Also for PPP1R1B (NM_032192)
|The antiserum was produced against synthesized non-phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).|
|Human, Mouse, Rat
||WB: 1:500~1000, IHC: 1:50~100, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|DARPP-32 (Ab-75) antibody detects endogenous levels of total DARPP-32 protein.
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 1|
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Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 30min), using DARPP-32 (Ab-75) antibody (#TA312428, Line 1 and 2).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using DARPP-32 (Ab-75) antibody (#TA312428).The picture on the right is treated with the synthesized peptide.