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Anti-PPP1R1B PHOSPHO Antibody
Also for PPP1R1B (NM_181505)
|The antiserum was produced against synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 34 (R-P-TP-P-A).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|DARPP-32 (Phospho-Thr34) antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 34.
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 2|
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Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using DARPP-32 (Phospho-Thr34) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using DARPP-32 (Phospho-Thr34) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using DARPP-32 (Phospho-Thr34) antibody.The picture on the right is treated with the synthesized peptide.