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Anti-PPP1R1B PHOSPHO Antibody
Also for PPP1R1B (NM_138521)
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH|
||0.5~1.0 mg/ml (Lot Dependent)
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
|Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b)|
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Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: Î»-Ptase). The blot is identical to the control except that it was incubated in Î»-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment with Î»-Ptase.