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Home Antibody All anti-PPP1R1B antibodies

Anti-PPP1R1B Antibody EP720Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300630
  • Rabbit Monoclonal Antibody against PPP1R1B (Clone EP720Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC403149) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(3)
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Also for PPP1R1B (NM_032192)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-term of human DARPP-32 was used as immunogen.
Clone NameEP720Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsIHC-Fr: 1:7500; ICC/IF: Use at an assay dependent concentration; WB: 1:5000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC: 1:50 - 1:100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 1
Alternative NameDARPP-32; DARPP32
Database LinkNP_115568
FunctionThe 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).
Related Pathway

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WB Image
Western blot - DARPP32 antibody [EP720Y]; Anti-DARPP32 antibody [EP720Y] at 1/50000 dilution + Rat brain lysate at 10 µg.Predicted band size : 32 kDa.Observed band size : 32 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - DARPP32 antibody [EP720Y]; Immunohistochemical analysis of human breast adenocarcinoma using TA300630.
IHC Image
Immunohistochemistry (Frozen sections) - DARPP32 antibody [EP720Y]; TA300630 staining DARPP32 in pig brain tissue sections by IHC-Fr (formaldehyde-fixed Frozen sections). Tissue was fixed with formaldehyde (4%), permeabilized with Triton X-100 and blocked with 0.2% milk for 30 minutes at 24°C. Samples were incubated with primary antibody (1/7500 in TBS + 1% Triton X-100 + 0.2% milk) for 24 hours at 4°C. A Biotin-conjugated Sheep polyclonal to rabbit IgG, dilution 1/400, was used as secondary antibody. Endogenous peroxidase was blocked with 0.3% H2O2 in methanol for 20 minutes.
IHC Image
IHC - Wholemount - Anti-DARPP32 antibody [EP720Y]; Immunohistochemical analysis of murine brain tissue, staining DARPP32 (red) with TA300630. Slices were fixed in paraformaldehyde and blocked with 5% horse serum and 0.25% Triton X-100. Samples were incubated with primary antibody (1/200) for 15 hours.

 

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