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Anti-PPP1R1A Antibody EP902Y
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Also for PPP1R1A (NM_006741)
|A synthetic peptide corresponding to residues in the N-terminus of human IPP-1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, IP, FC
||WB: 1:100000; IP: 1:50; FC: 1:20; ICC/IF: Use a concentration of 5 ug/ml; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 1A (PPP1R1A)|
Entrez Gene 5502 Human
Entrez Gene 58200 Mouse
Entrez Gene 58977 Rat
|The inhibitor of protein phosphatase 1 (IPP-1, I-1) is an endogenous inhibitor of protein phophastase 1 (PP-1), a signal transducer in many cellular pathways. IPP-1 is a member of heat-stable PP1 inhibitory subunits, which includes DARPP-32, (a homologue of IPP-1) and IPP-2. First discovered in rabbit skeletal muscle, IPP-1 is expressed in various mammalian tissues. IPP-1 inhibition of PP-1 is controlled through phosphorylation at Thr35 by cAMP-dependent protein kinase and a tetra-peptide consensus motif in various PP-1 binding proteins (1). IPP-1 is a hormonal control in muscle contraction, pituitary cell growth, synaptic plasticity by neurotransmitters, and glycogen metabolism (2). In vivo, IPP-1 is phosphorylated at Ser-67 by NCLK, which also initiates inhibition of PP-1 by IPP-1 (3). Triggered by increase in calcium level, IPP-1 is inactivated by PP2B through de-phosphorylation (4). |
|Druggable Genome Long-term potentiation|
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Western blot - Anti-Protein phosphotase inhibitor 1 antibody [EP902Y]; Anti-Protein phosphatase inhibitor 1 [EP902Y] antibody at 1/40000 dilution + Rat brain at 10 ug.Predicted band size : 19 kDa.Observed band size : 27 kDa .
Anti-Protein phosphotase inhibitor 1 antibody [EP902Y]; Immunohistochemical analysis of paraffin-embedded human normal brain tissue using TA300665 diluted 1:100.
ICC/IF image of TA300665 stained PC12 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Figure from citation: Immunofluorescence of PPP1R1A protein level by using anti-PPP1R1A antibody in STZ-injured rat ß-cells. Dilution: 1:800 View Citation
Figure from citation: Immunofluorescence of PPP1R1A protein level by using anti-PPP1R1A antibody in human pancreas. Dilution: 1:200 View Citation
Figure from citation: Immunoprecipitation of PPP1R1A protein level by using anti-PPP1R1A antibody in culture medium of rat islets. View Citation
Figure from citation: Immunoprecipitation of PPP1R1A protein level by using anti-PPP1R1A antibody in culture medium of human islets. View Citation
Flow Cytometry - Anti-Protein phosphotase inhibitor 1 antibody; Overlay histogram showing SH-SY5Y cells stained with TA300665 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.