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Anti-PPP1R15A TRUEMAB Antibody Clone OTI1E1
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for PPP1R15A (NM_014330)
|Full length human recombinant protein of human PPP1R15A(NP_005145) produced in HEK293T cell.|
|WB, IHC, FC
||WB 1:2000, IHC 1:150, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens protein phosphatase 1 regulatory subunit 15A (PPP1R15A)|
Entrez Gene 23645 Human
|This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008].|
|Druggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PPP1R15A monoclonal antibody.(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)
HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504359), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504359),(Red), compared to a nonspecific negative control antibody,(Blue).