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Anti-PPP1R14A Antibody E305
Also for PPP1R14A (NM_033256)
|A synthetic peptide corresponding to residues in the N-term of human CPI-17 aplha was used as immunogen. This antibody may also detect isoform CPI-17 beta according to sequence analysis.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500~1000; IHC: 1:250-500; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A), transcript variant 1|
|CPI-17; CPI17; PPP1INL|
|PKC Potentiated Inhibitor Protein-17 (CPI-17) is a 17 kDa protein (1). Expressed in smooth muscles and neuronal cells, CPI-17 is a myosin phosphatase inhibitor protein. Phosphorylation of CPI-17 at Thr38 enhances its inhibitory effect by 100 fold (2). Once phosphorylated, CPI-17 inhibits PP1c and MLCP holoenzyme activity (3). |
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Western blot - CPI17 alpha antibody [E305]; All lanes : Anti-CPI17 alpha antibody [E305] at 1/5000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : MCF-7 cell lysate.Lane 4 : A431 cell lysate .Predicted band size : 17 kDa.Observed band size : 17 kDa.
Immunohistochemistry (Paraffin-embedded sections) - CPI17 alpha antibody [E305]; ab32213,at a 1/250 dilution, staining human normal aorta by immunohistochemistry, Paraffin embedded tissue