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Anti-PPP1CA PHOSPHO Antibody EP1512Y
Also for PPP1CA (NM_206873)
|A phospho specific peptide corresponding to residues surrounding Threonine 320 of human PP1 alpha was used as an immunogen. This antibody detects PP1 alpha phosphorylated at Threonine 320.|
|Mouse, Rat, Human, Xenopus laevis
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:40; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 2|
|PP-1A; PP1A; PP1alpha; PPP1A|
Entrez Gene 5499 Human
Entrez Gene 19045 Mouse
Entrez Gene 24668 Rat
|Protein phosphatase 1 (PP1) is a major eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular functions through the interaction of its catalytic subunit (PP1c) with over fifty different established or putative regulatory subunits (1). The coordinated and reciprocal action of serine/threonine protein kinases and phosphatases produces transient phosphorylation, a fundamental regulatory mechanism for many biological processes. PP1 is ubiquitously distributed and regulates a broad range of cellular functions, including glycogen metabolism, cell-cycle progression and muscle relaxation. PP1 has evolved effective catalytic machinery but lacks substrate specificity. Substrate specificity is conferred upon PP1 through interactions with a large number of regulatory subunits (2). It has been shown that PP1 determines the efficacy of learning and memory by limiting acquisition and favouring memory decline. When PP1 is genetically inhibited during learning, short intervals between training episodes are sufficient for optimal performance (3). |
|PhosphataseDruggable Genome Oocyte meiosisVascular smooth muscle contractionFocal adhesionLong-term potentiationRegulation of actin cytoskeletonInsulin signaling pathwayMore Pathways >> |
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Western blot - PPP1A (phospho T320) antibody [EP1512Y]; All lanes : Anti-PPP1A (phospho T320) antibody [EP1512Y].Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate (treated with Calyculin A).Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP conjugate at 1/2000 dilution.Predicted band size : 38 kDa.Observed band size : 38 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PPP1A (phospho T320) antibody [EP1512Y]; Ab62334 (1/100-1/250) staining human PP1A in human muscle tissue by immunohistochemistry using paraffin embedded tissue.