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Home Antibody All anti-PPME1 antibodies

Anti-PPME1 TRUEMAB Antibody Clone OTI7F12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500823
  • anti-PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402509) , 20ug Explanation
100ul $379 In Stock
CF500823
  • Carrier-free (BSA/glycerol-free) PPME1 mouse monoclonal antibody, clone OTI7F12 (formerly 7F12)
100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(1)
IF(1)
FC(2)
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Also for PPME1 (NM_016147)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.
Clone NameClone OTI7F12 IsotypeIgG1
Species ReactivityHuman Concentration0.96 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 42.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens protein phosphatase methylesterase 1 (PPME1), transcript variant 1
Alternative NamePME-1
Database LinkNP_057231
Entrez Gene 51400 Human
FunctionProtein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500823, Dilution 1:50)
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Anti-PPME1 mouse monoclonal antibody (TA500823) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1(RC200009).
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Flow cytometric analysis of Hela cells, using anti-PPME1 antibody(TA500823),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody(TA500823),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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