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Anti-PPAT TRUEMAB Antibody Clone OTI1B11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504706 PPAT mouse monoclonal antibody, clone OTI1B11 (formerly 1B11) 100ul $325 In Stock
LC400951 PPAT HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504706 Carrier-free (BSA/glycerol-free) PPAT mouse monoclonal antibody, clone OTI1B11 (formerly 1B11) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(8)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.
Clone NameClone OTI1B11 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:500, IHC 1:150,
Predicted MW Explanation 57.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phosphoribosyl pyrophosphate amidotransferase (PPAT)
Alternative NameATASE; GPAT; PRAT
Database LinkNP_002694
Entrez Gene 5471 Human
FunctionThe protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011].
Related PathwayProteaseDruggable Genome Purine metabolismAlanine, aspartate and glutamate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (RC201144, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504706)
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