VEGFA Rabbit Polyclonal Antibody

CAT#: PP1073B1

VEGFA rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using VEGF-A Antibody Cat.-No [PP1073B]

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  Sandwich ELISA using VEGF-A Antibody Cat.-No [PP1073B]

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human VEGF by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at ~1.0 µg/ml. This Biotin conjugated antiboy allows the detection of 0.2-0.4 ng/well of recombinant Human VEGF.
  Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antiboy in conjunction with purified Cat.-No PP1073P as Capture antibody allows the detection of 2000-4000 pg/ml of recombinant Human VEGF.
  Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human VEGF (Cat.-No PA136).
• Specificity: This antibody reacts with Human Vascular Endothelial Growth Factor. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (Sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: vascular endothelial growth factor A
• Database Link:
  Entrez Gene 7422 Human
  UniProt ID P15692
• Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
• Note: Centrifuge vial prior to opening!