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Anti-POU5F1 TRUEMAB Antibody Clone OTI7H1
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for POU5F1 (NM_002701)
|Full length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.|
|Human , Rat , Mouse
|| WB 1:200~1000, IF: 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1|
|Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4|
Entrez Gene 5460 Human
Entrez Gene 18999 Mouse
Entrez Gene 294562 Rat
|This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]. |
|Stem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU5F1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POU5F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-POU5F1 mouse monoclonal antibody (TA506798) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1(RC211998).
Immunofluorescent staining of CRL1973 cells using anti-POU5F1 mouse monoclonal antibody (TA506798).