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Home Antibody All anti-POU5F1 antibodies

Anti-POU5F1 TRUEMAB Antibody Clone OTI7H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506798 POU5F1 mouse monoclonal antibody, clone OTI7H1 (formerly 7H1) 100ul $325 In Stock
LC400950 POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506798 Carrier-free (BSA/glycerol-free) POU5F1 mouse monoclonal antibody, clone OTI7H1 (formerly 7H1) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(2)
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Also for POU5F1 (NM_002701)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.
Clone NameClone OTI7H1 IsotypeIgG1
Species ReactivityHuman , Rat , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:200~1000, IF: 1:100
Predicted MW Explanation 38.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1
Alternative NameOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Database LinkNP_002692
Entrez Gene 5460 Human
Entrez Gene 18999 Mouse
Entrez Gene 294562 Rat
FunctionThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010].
Related PathwayStem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU5F1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POU5F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Anti-POU5F1 mouse monoclonal antibody (TA506798) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POU5F1(RC211998).
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Immunofluorescent staining of CRL1973 cells using anti-POU5F1 mouse monoclonal antibody (TA506798).
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