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Home Antibody All anti-OCT4 antibodies

Anti-OCT4 TRUEMAB Antibody Clone OTI9B7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500035 OCT4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7) 100ul $379 In Stock
LC400950 POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500035 Carrier-free (BSA/glycerol-free) OCT4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(3)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.
Clone NameClone OTI9B7 IsotypeIgG1
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:5000, IHC 1:50, IF 1:100,
Predicted MW Explanation 38.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1
Alternative NameOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Database LinkNP_002692
Entrez Gene 5460 Human
FunctionThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Related PathwayStem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-OCT4 monoclonal antibody at 1:200 dilution.
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
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Anti-OCT4 mouse monoclonal antibody (TA500035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT4(RC211998).
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