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Anti-OCT4 TRUEMAB Antibody Clone OTI9B7
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for OCT4 (NM_002701)
|Full length human recombinant protein of human POU5F1 (NP_002692) produced in E.coli.|
|WB, IHC, IF
||WB 1:5000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1|
|Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4|
Entrez Gene 5460 Human
|This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.|
|Stem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-OCT4 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
Anti-OCT4 mouse monoclonal antibody (TA500035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT4(RC211998).