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Home Antibody All anti-POU5F1 antibodies

Anti-POU5F1 Antibody

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SKU Description Amount Price Availability*  
TA319164 Rabbit polyclonal anti-Oct-4 antibody 100ug $325 3-7 Days
LC400950 POU5F1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, monkey, horse Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:350,000, WB: 1:1,000 - 1:1,500, IF: User Optimized, IP: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression; the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.

Reference Data

Target NameHomo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 3
Alternative NameOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Database LinkNP_001167002
Entrez Gene 5460 Human
Entrez Gene 714760 Monkey
Function
Related PathwayStem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using Anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
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