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Home Antibody All anti-POU5F1 antibodies

Anti-POU5F1 Antibody EPR2054

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307418
  • Rabbit monoclonal antibody against Oct-4(clone EPR2054)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400950) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(9)
Assay(1)
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Also for POU5F1 (NM_002701)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Oct-4 was used as an immunogen.
Clone NameEPR2054 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:500 - 1:1000; FC: 1:10 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC.

Reference Data

Target NameHomo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1
Alternative NameOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Database LinkNP_002692
Entrez Gene 5460 Human
FunctionOctamer transcription factor-4 (Oct-4) is a homeodomain transcription factor in the POU transcription factor family that specifically binds to the octamer motif (5’-ATTTCAT-3’) (1). It is critical for early embryogenesis and for embryonic stem cell pluripotency, expressed mainly in the developing brain (1). OCT4 is a master regulator that affects the fate of pluripotent stem cells and germ cell precursors (2). For instance, the downregulation of Oct-4 triggers embryonic stem cell differentiation, simultaneous with gastrulation (3). It is also used as a marker for germ cell tumor diagnosis (4).
Related PathwayStem cell - PluripotencyAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Oct4 antibody [EPR2054]; All lanes : Anti-Oct4 antibody [EPR2054] at 1/1000 dilution.Lane 1 : Seminoma lysate.Lane 2 : NCCIT cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 39 kDa.
Assay Image
Other-Anti-Oct4 antibody [EPR2054](TA307418); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Oct4 antibody [EPR2054]; TA307418, at 1/500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing negative staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing negative staining in Skeletal muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing negative staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing positive staining in Seminoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing positive staining in Fetal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing positive staining in Embryonal carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Oct4 antibody [EPR2054](TA307418); TA307418 showing negative staining in Hepatocellular carcinoma tissue.

 

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