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Anti-POU4F1 Antibody EP1972Y
Also for POU4F1 (NM_006237)
|A synthetic peptide corresponding to residues near the C-terminus of human POU domain protein 3a was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500 - 1:1000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; FC: 1:90; IP: 1:50; IHC - Wholemount: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens POU class 4 homeobox 1 (POU4F1)|
|brn-3A; BRN3A; Oct-T1; RDC-1|
|BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM]. |
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Western blot - BRN3A antibody [EP1972Y]; All lanes : Anti-BRN3A antibody [EP1972Y].Lane 1 : LNCaP.Lane 2 : SW480.Lane 3 : PC-3.Lane 4 : Human brain lysate.Predicted band size : 43 kDa.Observed band size : 43 kDa.
Western blot - BRN3A antibody [EP1972Y]; All lanes : Anti-BRN3A antibody [EP1972Y] at 1/2000 dilution.Lane 1 : Adult mouse cerebellum lysate.Lane 2 : Adult mouse lower brainstem lysate.Lysates/proteins at 20 µg per lane.Secondary.HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution.Performed under reducing conditions.Predicted band size : 43 kDa.Observed band size : 43 kDa.Exposure time : 2 minutesThis image is courtesy of an Anonymous Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BRN3A antibody [EP1972Y]; Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 ab81213.
Immunocytochemistry/ Immunofluorescence - BRN3A antibody [EP1972Y]; Immunofluorescent staining of HeLa cells using 1/100 ab81213.
Flow Cytometry-Anti-BRN3A antibody [EP1972Y](ab81213); Overlay histogram showing SH-SY5Y cells stained with ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.