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Home Antibody All anti-POLR2J2 antibodies

Anti-POLR2J2 TRUEMAB Antibody clone 3G4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502454
  • POLR2J2 mouse monoclonal antibody, clone 3G4
  • FREE positive control: HEK293T cell transient overexpression lysate (LC409843) , 20ug
100ul $325 In Stock
CF502454
  • Carrier-free (BSA/glycerol-free) POLR2J2 mouse monoclonal antibody, clone 3G4
100ug $450 3-4 weeks
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for POLR2J2 (NM_032959)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.
Clone Nameclone 3G4 IsotypeIgG2a
Species ReactivityHuman Concentration0.72 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2)
Alternative NameHRPB11B; RPB11b1
Database LinkNP_116581
FunctionThis gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq].
Related Pathway

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502454)
IF Image
Anti-POLR2J2 mouse monoclonal antibody (TA502454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2(RC224755).
FC Image
HEK293T cells transfected with either RC224755 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2J2 antibody(TA502454), and then analyzed by flow cytometry.

 

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