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Home Antibody All anti-POLR2E antibodies

Anti-POLR2E TRUEMAB Antibody Clone 3B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502548
  • POLR2E mouse monoclonal antibody,clone 3B5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419161) , 20ug
100ul $379 In Stock
CF502548
  • Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody,clone 3B5
100ug $450 3-4 weeks
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WB(2)
IHC(8)
FC(2)
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Also for POLR2E (NM_002695)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Clone NameClone 3B5 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.9mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)
Alternative NamehRPB25; hsRPB5; RPABC1; RPB5; XAP4
Database LinkNP_002686
FunctionThis gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502548)
FC Image
HEK293T cells transfected with either RC201266 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2E antibody(TA502548), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-POLR2E antibody(TA502548),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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