OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-POLR2E antibodies

Anti-POLR2E TRUEMAB Antibody Clone 3B5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502548
  • POLR2E mouse monoclonal antibody,clone 3B5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419161) , 20ug Explanation
100ul 379 In Stock
CF502548
  • Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody,clone 3B5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
Add to Shopping Cart
spacer
WB(2)
IHC(8)
FC(2)
spacer
Also for POLR2E (NM_002695)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Clone NameClone 3B5 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.9 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 24.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)
Alternative NamehRPB25; hsRPB5; RPABC1; RPB5; XAP4
Database LinkNP_002686
Entrez Gene 5434 Human
Entrez Gene 66420 Mouse
Entrez Gene 690966 Rat
Entrez Gene 611859 Dog
FunctionThis gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].
Related PathwayTranscription Factors Purine metabolismPyrimidine metabolismMetabolic pathwaysRNA polymeraseHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-POLR2E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502548)
FC Image
HEK293T cells transfected with either RC201266 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-POLR2E antibody(TA502548), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-POLR2E antibody(TA502548),(Red), compared to a nonspecific negative control antibody,(Blue).

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title