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Home Antibody All anti-POLR2E antibodies

Anti-POLR2E TRUEMAB Antibody Clone OTI3C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502488 POLR2E mouse monoclonal antibody, clone OTI3C5 (formerly 3C5) 100ul $325 In Stock
LC419161 POLR2E HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502488 Carrier-free (BSA/glycerol-free) POLR2E mouse monoclonal antibody, clone OTI3C5 (formerly 3C5) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IF(1)
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Also for POLR2E (NM_002695)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.
Clone NameClone OTI3C5 IsotypeIgG2a
Species ReactivityHuman Concentration0.74 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:500~2000, IF 1:100,
Predicted MW Explanation 24.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)
Alternative NamehRPB25; hsRPB5; RPABC1; RPB5; XAP4
Database LinkNP_002686
Entrez Gene 5434 Human
FunctionThis gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq].
Related PathwayTranscription Factors Purine metabolismPyrimidine metabolismMetabolic pathwaysRNA polymeraseHuntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.
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Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E(RC201266).
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