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Home Antibody All anti-PNPO antibodies

Anti-PNPO TRUEMAB Antibody Clone OTI1H9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503356 PNPO mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ul $325 In Stock
LC413282 PNPO HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503356 Carrier-free (BSA/glycerol-free) PNPO mouse monoclonal antibody, clone OTI1H9 (formerly 1H9) 100ug $450 3-4 weeks
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WB(2)
IHC(4)

OriGene Data

ImmunogenFull length human recombinant protein of human PNPO(NP_060599) produced in HEK293T cell.
Clone NameClone OTI1H9 IsotypeIgG2b
Species ReactivityHuman Concentration0.32 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:500, IHC 1:150,
Predicted MW Explanation 29.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens pyridoxamine 5'-phosphate oxidase (PNPO)
Alternative NameHEL-S-302; PDXPO
Database LinkNP_060599
Entrez Gene 55163 Human
FunctionThe enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway Vitamin B6 metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNPO (RC200133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNPO.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PNPO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PNPO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503356)
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