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Home Antibody All anti-PNMT antibodies

Anti-PNMT TRUEMAB Antibody clone 2D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502826
  • PNMT mouse monoclonal antibody, clone 2D5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400946) , 20ug
100ul $325 In Stock
CF502826
  • Carrier-free (BSA/glycerol-free) PNMT mouse monoclonal antibody, clone 2D5
100ug $450 3-4 weeks
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WB(1)
IHC(1)
IF(2)
FC(3)
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Also for PNMT (NM_002686)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PNMT (NP_002677) produced in HEK293T cell.
Clone Nameclone 2D5 IsotypeIgG2a
Species ReactivityHuman Concentration0.70 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1
Alternative NamePENT; PNMTase
Database LinkNP_002677
FunctionThe product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT.
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502826)
IF Image
Anti-PNMT mouse monoclonal antibody (TA502826) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT(RC206586 ).
IF Image
Immunofluorescent staining of HeLa cells using anti-PNMT mouse monoclonal antibody (TA502826).
FC Image
HEK293T cells transfected with either RC206586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PNMT antibody(TA502826), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody(TA502826),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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