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Anti-SILV TRUEMAB Antibody Clone OTI10E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500424 anti-SILV (Melanoma gp100) mouse monoclonal antibody, clone OTI10E5 (formerly 10E5) 100ul
30ul
$325 In Stock
LC402062 PMEL HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500424 Carrier-free (BSA/glycerol-free) SILV mouse monoclonal antibody, clone OTI10E5 (formerly 10E5) 100ug $450 3-4 weeks
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WB(3)
IHC(15)
IF(1)
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OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human SILV expression vector
Clone NameClone OTI10E5 IsotypeIgG2b
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.2 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:1000 IHC 1:50 IF 1:100 Flow 1:500
Predicted MW Explanation 70.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens premelanosome protein (PMEL), transcript variant 3
Alternative NameD12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
Database LinkNP_008859
Entrez Gene 6490 Human
Entrez Gene 362818 Rat
Entrez Gene 710921 Monkey
Entrez Gene 481102 Dog
FunctionCould be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity
Related PathwaySecreted ProteinTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SILV (RC200663, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SILV .
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WB Image
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-SILV monoclonal antibody at 1:200 dilution.
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WB Image
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SILV monoclonal antibody.(1:200)
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IHC Image
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SILV mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500424, Dilution 1:50)
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IF Image
Anti-SILV mouse monoclonal antibody (TA500424) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SILV (RC200663).
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