TEM7 (PLXDC1) Mouse Monoclonal Antibody [Clone ID: 197C193 (IM193)]

CAT#: TA336403

Mouse Monoclonal TEM7/PLXDC1 Antibody (197C193 (IM193))

Product Images

Immunohistochemistry-Paraffin: TEM7/PLXDC1 Antibody (197C193 (IM193)) TA336403 - Formalin-fixed, paraffin-embedded human breast vessel stained with TEM7 antibody at 5 ug/ml.

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  Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) TA336403 - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin counterstain.

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  Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) TA336403 - Detection of TEM7 with TEM7 antibody. Human HCT-116 cell lysate probed with TEM7 antibody at 1 ug/ml.

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  Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) TA336403 - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to NP_065138.2).

Specifications

Product Data

• Clone Name: 197C193 (IM193)
• Applications: ICC/IF, IHC, IP, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry-Frozen), Immunohistochemistry-Paraffin: 2-5 ug/ml, Immunoprecipitation: 1:10-1:500, Immunohistochemistry: 1:10-1:500, Western Blot
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: plexin domain containing 1
• Database Link:
  NP_065138
  Entrez Gene 72324 MouseEntrez Gene 303505 RatEntrez Gene 57125 Human
  UniProt ID Q8IUK5
• Background: Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, primary cancers of lung, pancreas, breast, and brain. TEM7 is expressed specifically in endothelium of these cancers, whether primary or metastasis. The other six members of this family (TEM1, 3, 4, 5, 8, and 9) also show similar expression pattern in lung and brain tumors, and liver metastasis. Since most of the genes expressed differentially in tumor endothelium are also expressed during angiogenesis, these newly discovered genes might provide important resources for basic and clinical studies of human angiogenesis.
• Synonyms: TEM3; TEM7
• Note: Immunocytochemistry/Immunofluorescence: see Meng et al, 2007. Immunohistochemistry (frozen): see Lee et al, 2006. Immunohistochemistry (paraffin): 2-5 ug/ml; see Nanda et al, 2004 and Lee et al, 2005. Immunoprecipitation: see Nanda et al, 2004.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane