OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PLK1 antibodies

Anti-PLK1 TRUEMAB Antibody Clone OTI1A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500395 PLK1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ul $325 In Stock
LC417592 PLK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500395 Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ug $450 3-4 weeks
Add to Shopping Cart

spacer
WB(2)
IP(1)
IHC(9)

OriGene Data

ImmunogenFull length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.
Clone NameClone OTI1A2 IsotypeIgG2b
Species ReactivityHuman Concentration1.9 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:150, IP 2ug/500ul
Predicted MW Explanation 68.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
Entrez Gene 5347 Human
Function
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
spacer
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PLK1 monoclonal antibody.(1:200)
spacer
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
spacer
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title