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Home Antibody All anti-PLK1 antibodies

Anti-PLK1 TRUEMAB Antibody clone 1A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500395
  • PLK1 mouse monoclonal antibody, clone 1A2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417592) , 20ug Explanation
100ul 325 In Stock
CF500395
  • Carrier-free (BSA/glycerol-free) PLK1 mouse monoclonal antibody, clone 1A2
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IP(1)
IHC(9)
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Also for PLK1 (NM_005030)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.
Clone Nameclone 1A2 IsotypeIgG2b
Species ReactivityHuman Concentration1.9 mg/ml
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB 1:2000, IHC 1:150, IP 2ug/500ul
Predicted MW Explanation 68.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens polo-like kinase 1 (PLK1)
Alternative NamePLK; STPK13
Database LinkNP_005021
Entrez Gene 5347 Human
Function
Related PathwayProtein KinaseDruggable Genome Cell cycleOocyte meiosisProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500395)
IP Image
Immunoprecipitation(IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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