Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY ADFP (RC202500) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADFP.