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Anti-PLD2 PHOSPHO Antibody
Also for PLD2 (NM_002663)
|The antiserum was produced against synthesized phosphopeptide derived from human PLD2 around the phosphorylation site of tyrosine 169 (E-N-YP-L-N).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|PLD2 (Phospho-Tyr169) antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
|Homo sapiens phospholipase D2 (PLD2), transcript variant 1|
|choline phosphatase 2; phospholipase D2|
EGFR1 Signaling Pathway
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Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using PLD2 (Phospho-Tyr169) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody.The picture on the right is treated with the synthesized peptide.