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Anti-PKN1 Antibody EPR3238
Also for PKN1 (NM_002741)
|A synthetic peptide corresponding to residues near the C-terminus of human PKN1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens protein kinase N1 (PKN1), transcript variant 2|
|DBK; PAK-1; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1|
|Protein kinase N (PKN) is a fatty acid- and Rho-activated serine/threonine protein kinase involved in the regulation of cell motility by association with cytoskeletal components such as neurofilament and alpha-actinin (1). Serine/threonine-protein kinase N1 (PKN1) belongs to the protein kinase C superfamily. It is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The phosphorylation of PKN1 by PDK1 may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis (2).|
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Western blot - PKN antibody [EPR3238]; All lanes : Anti-PKN antibody [EPR3238] at 1/1000 dilution.Lane 1 : SH-SY-5Y cell lysates.Lane 2 : U937 cell lysates.Lane 3 : Jurkat cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 104 kDa.Observed band size : 120 kDa .
Flow Cytometry - PKN antibody [EPR3238]; Flow cytometric analysis of PKN in permeabilized Jurkat cells, using a 1/10 dilution of TA307411 (red) or a rabbit IgG (negative) (green).