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Home Antibody All anti-PIM2 antibodies

Anti-PIM2 TRUEMAB Antibody Clone OTI8H10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501070 anti-PIM2 mouse monoclonal antibody, clone OTI8H10 (formerly 8H10) 100ul $325 In Stock
LC416355 PIM2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501070 Carrier-free (BSA/glycerol-free) PIM2 mouse monoclonal antibody, clone OTI8H10 (formerly 8H10) 100ug $450 3-4 weeks
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WB(1)
IP(1)
IHC(4)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Clone NameClone OTI8H10 IsotypeIgG3
Species ReactivityHuman Concentration0.92 mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100, IP: 4ug/mL
Predicted MW Explanation 34kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Pim-2 proto-oncogene, serine/threonine kinase (PIM2)
Alternative Name
Database LinkNP_006866
Entrez Gene 11040 Human
FunctionThis gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.
Related PathwayProtein KinaseDruggable Genome Acute myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501070, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501070, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501070, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501070, Dilution 1:50)
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Anti-PIM2 mouse monoclonal antibody (TA501070) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2(RC201933).
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Immunoprecipitation(IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of
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HEK293T cells transfected with either pCMV6-ENTRY PIM2(RC201933)(Red) or empty vector control plasmid(Blue) were immunostained with anti-PIM2 mouse monoclonal(TA501070), and then analyzed by flow cytometry.
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