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Home Antibody All anti-PHKA1 antibodies

Anti-PHKA1 Antibody

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SKU Description Amount Price Availability*  
TA308404
  • Rabbit Polyclonal antibody to PHKA1 (phosphorylase kinase, alpha 1 (muscle))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC426544) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for PHKA1 (NM_001122670)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 828 and 1062 of PHKA1 (Uniprot ID#P46020)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat) Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 137kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Mouse (96%), Rat (95%)

Reference Data

Target NameHomo sapiens phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 2
Alternative NamePHKA
Database LinkNP_001116142
Entrez Gene 5255 Human
Entrez Gene 18679 Mouse
Entrez Gene 64561 Rat
FunctionThe PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]
Related PathwayDruggable Genome Calcium signaling pathwayInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (30 ug of whole cell lysate). A: HeLa. 7.5% SDS PAGE. TA308404 diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PHKA1(TA308404) antibody at 1:500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa, using PHKA1(TA308404) antibody at 1:200 dilution.
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