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Home Antibody All anti-PHB antibodies

Anti-PHB Antibody EP2804Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303636
  • Rabbit monoclonal antibody against Prohibitin (C-term) (EP2804Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419205) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for PHB (NM_002634)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human prohibitin was used as an immunogen.
Clone NameEP2804Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:500 - 1:1000; IP: 1:20; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens prohibitin (PHB), transcript variant 2
Alternative NameHEL-215; HEL-S-54e; PHB1
Database LinkNP_002625
Entrez Gene 5245 Human
Entrez Gene 18673 Mouse
Entrez Gene 25344 Rat
FunctionProhibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Prohibitin antibody [EP2804Y]; All lanes : Anti-Prohibitin antibody [EP2804Y] at 1/1000 dilution.Lane 1 : MCF-7 cell lysates.Lane 2 : PC-12 cell lysates.Lane 3 : A431 cell lysates.Lane 4 : Ramos cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 30 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prohibitin antibody [EP2804Y]; TA303636 at 1/100 dilution staining Prohibitin in human hepatocellular carcinoma tissue, using a HRP/AP polymerized secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Prohibitin antibody [EP2804Y]; Immunofluorescent staining of HeLa cells using TA303636 at a 1/100 dilution.

 

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