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Home Antibody All anti-PFKP antibodies

Anti-PFKP TRUEMAB Antibody Clone OTI3H8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503999 PFKP mouse monoclonal antibody, clone OTI3H8 (formerly 3H8) 100ul $325 In Stock
LC400932 PFKP HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503999 Carrier-free (BSA/glycerol-free) PFKP mouse monoclonal antibody, clone OTI3H8 (formerly 3H8) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
Clone NameClone OTI3H8 IsotypeIgG1
Species ReactivityHuman Concentration0.6 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 85.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phosphofructokinase, platelet (PFKP), transcript variant 1
Alternative NameATP-PFK; PFK-C; PFK-P; PFKF
Database LinkNP_002618
Entrez Gene 5214 Human
FunctionThe PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].
Related PathwayDruggable Genome Glycolysis / GluconeogenesisPentose phosphate pathwayFructose and mannose metabolismGalactose metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (RC200673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKP.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503999)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503999)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503999)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503999)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503999)
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Flow cytometric Analysis of Hela cells, using anti-PFKP antibody(TA503999),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PFKP antibody(TA503999),(Red), compared to a nonspecific negative control antibody,(Blue).
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