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Home Antibody All anti-PDPK1 antibodies

Anti-PDPK1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324250
  • Anti-PDPK1 Rabbit Polyclonal Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400925) , 20ug
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenPeptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 3
Alternative NamePDK1; PDPK2; PRO0461
Database LinkNP_001248745
FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development
Related Pathway
Focal Adhesion
Hemostasis
Signaling by GPCR

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WB Image
Predicted band size: 63 kDa . Positive control: MCF, 293 and PC12 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: MCF, 293 and PC12 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PDK1 (Ab-241) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
IF Image
Predicted cell location: Cytoplasm; Nucleus; Cell membrane. Positive control: MCF7 cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed MCF7 cells using PDPK1 antibody at dilution 1/100. (Original magnification: ×200)

 

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