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Home Antibody All anti-PDPK1 antibodies

Anti-PDPK1 Antibody EPR245

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307428
  • Rabbit monoclonal antibody against PDK1(clone EPR245)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400925) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human PDK1 was used as an immunogen.
Clone NameEPR245 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 1
Alternative NamePDK1; PDPK2; PRO0461
Database LinkNP_002604
Entrez Gene 5170 Human
Entrez Gene 18607 Mouse
Entrez Gene 81745 Rat
FunctionPDK1 (3-Phosphoinositide-dependent protein kinase 1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).
Related PathwayProtein KinaseDruggable Genome PPAR signaling pathwaymTOR signaling pathwayFocal adhesionInsulin signaling pathwayEndometrial cancerProstate cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PDPK1 antibody [EPR245]; All lanes : Anti-PDPK1 antibody [EPR245] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : PC12 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 63 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 antibody [EPR245]; TA307428, at a 1/500 dilution, staining PDPK1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

 

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