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Anti-PDLIM1 Antibody EPR7187
Also for PDLIM1 (NM_020992)
|A synthetic peptide corresponding to residues in human PDLIM1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens PDZ and LIM domain 1 (PDLIM1)|
|CLIM1; CLP-36; CLP36; hCLIM1|
|PDLIM1, a member of the alpha-actinin-associated LIM protein (ALP)/enigma protein family, contains PDZ and LIM domains that are modular protein interaction motifs and mediate protein association with the cytoskeleton and proteins involved in signal transduction (1, 2). It associates with actin filaments and stress fibers in activated human platelets and endothelial cells (1). Moreover, PDLIM1 plays a role in neurite outgrowth in the peripheral nervous system (2).|
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Western blot - Anti-PDLIM1 antibody [EPR7187]; All lanes : Anti-PDLIM1 antibody [EPR7187] at 1/1000 dilution.Lane 1 : Jurkat cell line lysate.Lane 2 : HeLa cell line lysate.Lane 3 : 293T cell line lysate.Lane 4 : Caco 2 cell line lysate.Lane 5 : Saos 2 cell line lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 36 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1 antibody [EPR7187]; ab126628, at 1/250, staining PDLIM1 in HeLa cells by Immunofluorescence.